PCR和限制性内切酶法快速检测C57BL/ 6J ob 小鼠基因型

doi:10.3969/j.issn.1004-616x.2000.03.005

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2000, Vol. 12 ›› Issue (3): 143-145.doi: 10.3969/j.issn.1004-616x.2000.03.005

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RAPID DETECTION OF ob GENOTYPE BY PCR AND RESTRICTION ENDONUCLEASE

XU Pei-yu1 , Jerlin Walker2

1. Toxicologic Department , West China University of Medical Sciences , Chengdu 　610044 , China 　2.Penington Medical Research Center , L ousiana S tate University , Baton Rough , L A 　70808 , USA

Received:1999-07-20 Revised:1999-10-28 Online:2000-07-30 Published:2000-07-30

Abstract

Abstract: Purpose and Methods : The ob gene point mutant in C57BL/ 6J ob mice cause genetic obesity in all life. To improve the speed and efficiency of ob genotyping , we developed a method with PCR and rest riction endonuclease by property primer. Results and Conclusion : Three genotype (ob/ ob , ob/ + , + / + ) of the mouse are rapidly , differetiated with this assay. Since only a small biopsy specimen ( tail end) is needed to genotype ,the ob mouse at any age is typable and can be used in subsequent breeding or research.

Key words: ob mice genotype, PCR, rest riction endonuclease

CLC Number:

Q355

XU Pei-yu , Jerlin Walker. RAPID DETECTION OF ob GENOTYPE BY PCR AND RESTRICTION ENDONUCLEASE[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2000, 12(3): 143-145.

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RAPID DETECTION OF ob GENOTYPE BY PCR AND RESTRICTION ENDONUCLEASE

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